Effect of Vitamin D3 on Expression of Toll-like Receptor 4 in Intestinal Mucosa in Colitis Model in Rats
10.3969/j.issn.1008-7125.2017.04.008
- VernacularTitle:维生素D3对结肠炎大鼠肠黏膜Toll样受体4表达的影响
- Author:
Hongliang GAO
;
He ZHOU
;
Ping YAO
- Keywords:
Colitis;
Ulcerative;
Cholecalciferol;
Toll-Like Receptor 4
- From:
Chinese Journal of Gastroenterology
2017;22(4):229-232
- CountryChina
- Language:Chinese
-
Abstract:
Background: The prevalence of ulcerative colitis (UC) in China has significantly increased in recent years,and Toll-like receptor 4 (TLR4) may be closely related to the development of UC.Aims: To study the effect of vitamin D3 on expression of TLR4 in the intestinal mucosa in colitis model in rats.Methods: Thirty Sprague-Dawley rats were randomly divided into normal control group,model group and vitamin D3 group.Rats in model group and vitamin D3 group were given trinitro-benzene-sulfonic acid (TNBS) to induce colitis model.Rats in vitamin D3 group were given vitamin D3.HE staining was performed,and disease activity index (DAI) and colon histopathological score were evaluated,the expression of TLR4 was measured by immunohistochemistry.Results: Compared with normal control group,DAI and histopathological score in model group were significantly increased (P＜0.05),and expression of TLR4 was significantly increased (P＜0.05).After giving vitamin D3,DAI and histopathological score were significantly decreased (P＜0.05),and expression of TLR4 was significantly decreased (P＜0.05).Conclusions: The expression of TLR4 is increased in colon tissue in colitis model in rats,which may be involved in the pathogenesis of UC.Vitamin D3 can alleviate intestinal inflammation via inhibiting expression of TLR4,thereby playing a role in the adjunctive therapy of UC.
