Effect of ethanolic extract of Omithogalum caudatum Ait on apoptosis of Candida albicans
10.3969/j.issn.1005-1678.2017.04.003
- VernacularTitle:虎眼万年青提取物对白色念珠菌细胞凋亡的影响
- Author:
Nan WANG
;
Meizhu MENG
;
Kunpeng XIE
;
Mingjie XIE
- Keywords:
Omithogalum caudatum Ait;
Candida albicans;
cell apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(4):9-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Omithogalum caudatum Ait(OCA)on apoptosis of Candida albicans,illustrated the antifungal mechanism of OCA.Methods Annexin Ⅴ-FITC/PI double stainingwas used to detect the effect of OCA on the apoptosis of C.albicans;JC-1 and DCFH-DA staining were used to detectthe effect of OCA on mitochondrial membrane potential(MTP)and reactive oxygen species(ROS)of C.albicans.Results OCA had a good antifungal activity,the minimum inhibitory concentration(MIC)and the minimum fungicidal concentration(MFC)were 8mg/mL and 32mg/mL respectively.OCA could induceapoptosis of C.albicans,promote the reduction of MTP and increase of ROS.Conclusion OCA induced cell apoptosismainly through disrupting mitochondrial function.
