Molecular identification and typing of 182 strains of Streptococcus pneumoniae isolated from children
10.3969/j.issn.1671-9638.2017.04.009
- VernacularTitle:182株儿童患者肺炎链球菌的分子生物学鉴定及分型
- Author:
Yinghui GUO
;
Baohua HE
;
Yingtong WANG
;
Zhaoyi JIA
;
Qian WANG
;
Guixia LI
;
Wenchao ZHANG
;
Yinqi SUN
;
Suliang CHEN
- Keywords:
Streptococcus pneumoniae;
multiplex polymerase chain reaction;
bacterial typing;
species identification;
cps gene
- From:
Chinese Journal of Infection Control
2017;16(4):326-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To type Streptococcus pneumoniae(S.pneumoniae) isolated from children, and provide scientific basis for the correct selection of S.pneumoniae vaccine.Methods 182 strains of S.pneumoniae were collected from Children's Hospital of Hebei Province in 2014, species of strains were identified by polymerase chain reaction (PCR), types of strains were analyzed with multiplex PCR.Results PCR detection showed that cpsA gene amplification of 182 strains were all positive;multiplex PCR detection revealed that except 8 strains were not typed, the main types of the remaining 174 strains were 19 F (n=68, 37.36%), 19A(n=33, 18.13%), and 6A/6B (n=26,14.28%), the other types were 35B, 14, 6C/6D, 23F, 15B/15C, and so on.Conclusion The main types of 182 strains of S.pneumoniae are 19 F, 19A, and 6A/6B, which provide scientific basis for the correct selection of S.pneumoniae vaccine for this province.
