Determination of the Entrapment Efficiency and Drug Loading Capacity of Curcumin and Quercetin Loaded Self-microemulsifying Drug Delivery System
- VernacularTitle:HPLC法测定姜黄素-槲皮素复方自微乳的载药量和包封率
- Author:
Ruixue HUANG
;
Yijun LI
;
Yuwei MAO
;
Lehuan LIU
;
Jianhong WANG
;
Jiaqi YU
;
Jundong DAI
- Keywords:
Curcumin;
Quercetin;
Self-microemulsifying drug delivery system;
HPLC;
Entrapment efficiency;
Drug loading
- From:
China Pharmacist
2017;20(4):664-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method to determine the entrapment efficiency (EE) and drug loading (DL) of curcumin (CUR)and quercetin (QUE)loaded self-microemulsifying drug delivery system.Methods:A centrifugation method was used to isolate the free drug.The content of drug was determined by HPLC.The analytical column was a Purospher STAR LP C18 column (250 mm×4.6 mm,5 μm) and the column temperature was 30 ℃.The mobile phase was acetonitrile-4% acetic acid (50∶50) and the flow rate was 1.0 ml·min-1.The UV detection wavelength was set at 370 nm and the injection volume was 10 μl.Results:CUR and QUE were linear within the range of 10.728-96.552 μg·ml-1 (r=0.999 8) and 1.08-9.72 μg·ml-1 (r=0.999 9),respectively.The average recovery was 99.98%(RSD=1.46%,n=9) and 100.34%(RSD=1.06%,n=9),respectively.In CUR-QUE-SMEDDS,the EE of curcumin and quercetin was (95.97±0.50)% and (95.91±2.52)%,and the DL was (25.82±0.15) mg·g-1 and (1.80±0.05)mg·g-1,respectively.Conclusion:The method is accurate,rapid and simple,and suitable for the determination of DL and EE in CUR-QUE-SMEDDS.
