Screening and Optimization of the Extraction Technology of Total Flavonoids in Persimmon Leaves by Semi-bionic-enzyme Method
10.6039/j.issn.1001-0408.2017.13.24
- VernacularTitle:半仿生酶法提取柿叶中总黄酮的工艺筛选及优化
- Author:
Xuanji XUE
;
Jun LUO
;
Xinxin ZHANG
;
Chunxiang QIAN
;
Jing ZHAO
;
Zengjun GUO
- Keywords:
Semi-bionic-enzyme method;
Persimmon leaves;
Favonoids;
Extraction;
Orthogonal test;
Technology optimization
- From:
China Pharmacy
2017;28(13):1813-1816
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen the method for extracting total flavonoids in persimmon leaves and optimize extraction tech-nology. METHODS:Using extract quality and flavonoids content as indexes,the effects of extracting total flavonoids in persim-mon leaves by ethanol refluxing method,enzyme method(cellulase,β-glucanase and complex enzyme mixed by equal amounts of both),semi-bionic method and semi-bionic-enzyme method (the same enzymes) were compared. Using flavonoids content as in-dex,solid-liquid radio,reflux temperature,reflux time as factors,orthogonal test was designed to optimize the extraction technolo-gy conditions of flavonoids in persimmon leaves by semi-bionic-enzyme method,and the verification test was conducted. RE-SULTS:The extract quantity and flavonoids content by semi-bionic-enzyme method was the highest among the 4 extraction meth-ods,and the complex enzyme was the most suitable;the optimized extracting condition of semi-bionic-enzyme method were as fol-lows as solid-liquid radio of 1:14,reflux temperature of 50 ℃,reflux time of 2.0 h;extraction rates of flavonoids in 3 verification tests were 5.9%,5.8%,5.9%(RSD=0.98%,n=3). CONCLUSIONS:The optimized semi-bionic-enzyme method is efficient and stable in extracting flavonoids in persimmon leaves.
