Study on the Extraction Technology of Asiaticosides from Centella asiatica by Ultrasonic-enzyme Method
10.6039/j.issn.1001-0408.2017.13.25
- VernacularTitle:超声波-酶法提取积雪草中积雪草总苷的工艺研究
- Author:
Dandan ZHANG
;
Xuqiang NIE
;
Han ZHANG
;
Jiufeng ZHAO
;
Xiujun SHI
;
Jianwen YANG
- Keywords:
Centella asiatica;
Asiaticoside;
Hydroxy asiaticoside;
Ultrasonic;
Cellulose;
Extraction technology;
Orthogonal test
- From:
China Pharmacy
2017;28(13):1816-1819
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of asiaticosides from Centella asiatica. METHODS:Using to-tal amounts of asiaticoside and hydroxy asiaticoside as investigation indexes,single factor and orthogonal test were used to investi-gate the enzyme amount,enzymolysis time,enzymolysis temperature,ethanol volume fraction,liquid material ratio and ultrasonic extraction time and optimize the extraction technology of asiaticosides from C. asiatica by ultrasonic-enzyme method,and verifica-tion test was conducted. RESULTS:Optimal extraction technology was as follow as cellulose dosage of 12 mg/g,10-fold liquid ma-terial ratio added into 60% ethanol,enzyme hydrolysis for 60 min at 60 ℃,ultrasonic assisted extraction for 50 min. Average ex-traction rate of total asiaticosides was 1.92%(RSD=1.83%,n=3)in verification test. CONCLUSIONS:Ultrasonic-enzyme meth-od is stable and feasible for the extraction technology of asiaticosides from C. asiatica.
