Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Erxieting Granule by HPLC
- VernacularTitle:HPLC法同时测定儿泻停颗粒中甘草苷和甘草酸的含量
- Author:
Qingxia CHU
;
Haijun QIN
;
Junling LIU
;
Hui ZHANG
;
Bo YU
;
Yuhan MA
;
Yazhong ZHANG
- Keywords:
Erxieting granules;
Liquiritin;
Glycyrrhizic acid;
HPLC
- From:
China Pharmacist
2017;20(4):743-745
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of liquiritin and glycyrrhizic acid in Erxieting granule.Methods:A TechMate C18-ST(250 mm×4.6 mm,5 μm) column with a DAD detector was used.The mobile phase consisted of acetonitrile (A) and 0.05% phosphoric acids in water (B) with gradient elution.The flow rate was 1.0 ml·min-1 and the detection wavelength was 237 nm.The sample size was 5 μl and the column temperation was room temperatence.Results:Linear calibration curves were obtained within the range of 10.32-51.62 mg·L-1 for liquiritin and 79.40-397.00 mg·L-1for glycyrrhizic acid.The average spiked recovery of liquiritin and glycyrrhizic acid was 98.10(RSD=1.0%,n=6)and 97.15(RSD=1.8%,n=6),respectively.Conclusion:The method is accurate,reproducible and stable,and can be used for the quality control of Erxieting granule.
