Determination of Triptolide in Shenshe Ointment by LC-MS/MS
- VernacularTitle:液相色谱-质谱联用法测定参蛇软膏中雷公藤甲素的含量
- Author:
Zhiqiang MENG
;
Shanbo MA
;
Likun DING
;
Shan MIAO
;
Yin WU
;
Jinyi CAO
;
Long LI
;
Biyan DANG
;
Xiaopeng SHI
- Keywords:
Shenshe ointment;
LC-MS/MS;
Content determination;
Triptolide
- From:
China Pharmacist
2017;20(4):745-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an LC-MS/MS method for the content determination of triptolide in Shenshe ointment.Methods:The chromatographic separation was performed on an Agilent TC-C18 (250 mm×4.6 mm,5 μm)column with the mobile phase consisting of methanol-0.1% formic acid solution (73∶27,v/v).The flow rate was 0.5 ml·min-1 and the column temperature was maintained at 35℃.An electrospray ionization (ESI) source was applied with multiple reaction monitoring (MRM) combined with a positive mode.Metronidazole was used as the internal standard.The mass transitions were 361.4→43.2 for triptolide and 172.1→128.0 for metronidazole,respectively.Results:The linear range was 10-500 ng·ml-1 with good correlation coefficient (r=0.999 9).The limit of quantification (LOQ) was 9.5 ng·ml-1.The intra-and inter-day RSDs of peak areas were all less than 3% and the average recovery was 96.87%(RSD=2.79,n=6).Conclusion:The established LC-MS/MS method is simple,efficient,sensitive and accurate in the quality control of Shenshe ointment.
