Quality Standard for Qingchang Mixture
- VernacularTitle:清肠合剂质量标准的建立
- Author:
Ting LI
;
Liu YANG
;
Yisheng ZHANG
;
Wei WANG
;
Xiaojing SUN
;
Li LI
;
Xiaoxia XIE
- Keywords:
Qingchang mixture;
Quality standard;
Ferulic acid;
HPLC;
Content determination
- From:
China Pharmacist
2017;20(4):748-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard for Qingchang mixture.Methods:TLC was adopted to identify angelica sinensis,paeoniae radix and phellodendri cortex.HPLC was used to determine the content of ferulic acid on a Wondasil C18 (250 mm×4.6 mm,5 μm) analytical column,the mobile phase was composed of acetonitrile-0.1% phosphoric acid solution(17∶83),the flow rate was 1.0 ml·min-1, and the detection wavelength was 316 nm and the column temperature was 30℃.Results:The TLC spots were clear and well-separated without any negative interference.The calibration curve of ferulic acid was in good linearity over the range of 39.6-316.8 μg·ml-1(r=0.999 9),the average recovery was 98.75% and RSD was 1.21%(n=9).Conclusion:The method is fast and accurate with good specificity,which can be used for the quality control of Qingchang mixture.
