Delphinidin induces autophagy in HER-2+ breast cancer cells via inhibition of AKT/mTOR pathway
10.11817/j.issn.1672-7347.2017.03.005
- VernacularTitle:飞燕草素通过AKT/mTOR通路诱导HER-2+乳腺癌细胞自噬
- Author:
Jingyao CHEN
;
Jie ZHOU
;
Fei LI
;
Yanfeng ZHU
;
Weiwei ZHANG
;
Xiaoping YU
- Keywords:
delphinidin;
breast cancer;
apoptosis;
autophagy;
AKT/mTOR
- From:
Journal of Central South University(Medical Sciences)
2017;42(3):264-270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of delphinidin on breast cancer and the underlying mechanisms.Methods:Human epidermal growth factor receptor-2 (HER-2) positive breast cancer cells MDA-MB-453 were treated by delphinidin.Proliferation of MDA-MB-453 cells was detected by CCK-8 after 48 h.TdT-mediated dUTP nick end labeling (TUNEL) assay and Western blot were used to explore apoptotic status for MDA-MB-453 cells.Fluorescence dot assay,immunofluorescence,and Western blot were used to identify autophagy in breast cancer cells.Results:Delphinidin suppressed proliferation of MDA-MB-453 cells.Delphinidin increased the number of TUNEL positive cells.Delphinidin downregulated the expression of caspase-3 and caspase-9,while upregulated the expression of cleaved caspase-3 and cleaved caspase-9 in a dose-dependent manner.Delphinidin enhanced the number of GFP-LC3 punctate dots,LC3 immunofluorescence dots and the expression of LC3-Ⅱ and ATG5.Delphinidin inhibited the expression of proteins in mTOR signaling pathway,induding AKT,mTOR,eIF4E and p70s6k.Conclusion:Delphinidin induced apoptosis and autophagy by inhibition of AKT/mTOR pathway in HER positive breast cancer cells.
