Effect of curcumin on Aβ production in swAPP HEK293 cells
10.3969/j.issn.1006-5725.2017.03.008
- VernacularTitle:姜黄素对swAPP HEK293细胞β样淀粉样蛋白生成的影响
- Author:
Qiwen GUO
;
Hongying LIU
;
Peng ZHOU
;
Shaoqing YANG
;
Qiulei CHEN
;
Hui ZHANG
;
Yanping WANG
- Keywords:
Curcumin;
Amyloid precursor protein;
β-amyloid;
miR-153;
Alzheimer's disease
- From:
The Journal of Practical Medicine
2017;33(3):363-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of curcumin on Aβ production in swAPP HEK293 cells and its preliminary mechanism.Methods swAPP HEK293 was used as cell model,the effect of curcumin (at different time points and of concentration) on cell viability was accessed by MTT assay.After the cells were treated with non-cytotoxic concentration of 5 μmol/L for different time,ELISA was used to detect Aβ production.The concentration and the time point of the strongest inhibitory effect were selected for the following tests.Real time PCR was employed to analyze miR-153 and APP mRNA expression,and Western blot was used to detect APP protein expression.Results As compared with the control group,curcumin of ≤ 5 μmol/L had no toxicity effect on the cell viability (P ＞ 0.05).Curcumin significantly inhibited Aβ production (P ＜ 0.05).Therefore 5 μmol/L curcumin and 24 h were selected as the best concentration and timc.Curcumin of 5 μmol/L had no obvious impact on APP mRNA expression (P ＜ 0.05),whereas markedly decreased APP protein expression.In addition,miR-153 level in the cells was significantly increased by 5 μmol/L curcumin treatment (P ＜ 0.05).Conclusion Curcumin may inhibit Aβ production through up-rcgulating miR-153 level and reducing APP protein expression in swAPP HEK293 cells.
