Effect of tripterygium glycosides contained serum on pathological boneforming related inflammatory markers and miR-21
10.3969/j.issn.1006-5725.2017.03.009
- VernacularTitle:雷公藤多苷含药血清对强直性脊柱炎病理性骨化相关炎症因子和miR-21的影响
- Author:
Yucong ZOU
;
Zheng MAO
;
Minpeng XU
;
Shaojun HU
;
Hongyu JIE
;
Junqi CHEN
;
Gang LIU
- Keywords:
Tripterygium glycosides;
Ankylosing spondylitis;
Pathological boneforming;
Inflammatory markers;
miR-21
- From:
The Journal of Practical Medicine
2017;33(3):367-370
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tripterygium glycosides (TG) contained serurn on the pathological boneforming related inflammatory markers and miR-21.Methods Previous isolated and cultured AS fibroblasts were stimulated using IL-1 of 1ng/ml for 24h,different concentrations of blank serum (5％,10％,and 15％) and TG contained serum (5％,10％,and 15％) were added for 48h.PGE-2,IL-17,IL-22,IL-23,CCL19 and CCL21 proteins were examined by Western blot.The osteogenesis marker BMP and microRNA-21 mRNAs were tested.Results 48 h after intervention,the expressions of inflammatory markers were obviously inhibited by TG contained serum;the boncforming related inflammatory markers,expressions of BMP-2 and miR-2t were all inhibited in a dose-dependent manner.Conclusions TG could inhibit the expressions of boneforming related inflammatory markers,BMP-2 and miR-21,thus providing theoretical basis to treat AS pathological boneforming.
