Complete genome analysis of influenza A(H1N1) pdm09 virus isolated from one case of pneumonia of unknown etiology (PUE) in Fujian Province, China
10.3969/j.issn.1002-2694.2017.03.007
- VernacularTitle:福建省1例不明原因肺炎病例中甲型H1N1流感病毒的全基因组特征分析
- Author:
Yanhua ZHANG
;
Yuwei WENG
;
Jianming ZHANG
;
Wenqiong XIU
;
Hongbin CHEN
;
Lin ZHAO
;
Wenxiang HE
;
Ying ZHU
;
Jianfeng XIE
;
Kuicheng ZHENG
- Keywords:
pneumonia of unknown etiology (PUE);
influenza A(H1N1) pdm09 virus;
characteristics of complete genome
- From:
Chinese Journal of Zoonoses
2017;33(3):228-235
- CountryChina
- Language:Chinese
-
Abstract:
To study the biological characteristics and mutations of influenza A(H1N1)pdm09 virus isolated from one case of pneumonia of unknown etiology (PUE),which would provide references for clinical treatment and disease control,the throat swab specimen from the PUE case was isolated in the Madin-Darby Canine Kidney (MDCK) cells,and then the antigenicity,pathogenicity and drug resistance of influenza A (H1N1) pdm09 virus were analyzed after sequencing.As a result,one influenza virus strain was isolated from the specimen and named as A/FujianGulou/SWL64/2016(H1N1).The similarities of nucleotide sequences and amino acids sequences compared with the vaccine strain A/California/07/2009 (H1N1) were 96.9％-98.9％ and 96.7％-99.5％,respectively.Eighteen amino acids had mutated in the HA and 4 mutations,K163Q,S185T,S203T and D222N,were involved in 3 different epitopes,which indicated that the antigenic drift had occurred in the influenza virus.The D222N mutation associated with receptor binding site made the virus infect lower respiratory tract more easily.The virus was still amantadine-resistance and oseltamivir-sensitive.In conclusion,the influenza A (H1N1) pdm09 virus in this study have occurred antigenic drift and has the molecular characterization of causing severe pneumonia,so further surveillance should be performed to prevent and control the influenza epidemic.
