Cloning, expression and purification of Nocardia brasiliensis proteion P61 with biological activity
10.3969/j.issn.1002-2694.2017.03.013
- VernacularTitle:克隆、表达和纯化具有生物学活性的巴西诺卡菌P61蛋白
- Author:
Xingzhao JI
;
Lu TANG
;
Xuexin HOU
;
Lina SUN
;
Chao WEI
;
Shuai XU
;
Chenchen SI
;
Zhenjun LI
- Keywords:
P61;
catalase;
prokaryotic expression
- From:
Chinese Journal of Zoonoses
2017;33(3):260-263
- CountryChina
- Language:Chinese
-
Abstract:
We constructed prokaryotic recombinant expression vector of P61 gene from Nocardia brasiliensis,expressing P61 protein with biological activity in E.coli,and lay a foundation for further studies related to P61.P61 gene was synthesized and cloned into an expression vector pET-30a(+).The recombinant vector was transformed into Escherichia coli BL21 and induced with IPTG.The production was analyzed with Western blot and the catalase activity of P61 was tested with Catalase Assay Kit.The protein of P61was successfully expressed in E.coli with solubility and high catalase activity,and could be identified by anti-N.brasiliensis sera from mice.The prokaryotic expression plasmid of protein P61 was constructed successfully and can be expressed efficiently in E.coli BL21 cells with higher catalase.
