Molecular characteristics of Listeria monocytogenes foodborne isolates in Hangzhou, China
10.3969/j.issn.1002-2694.2017.03.014
- VernacularTitle:杭州地区单核细胞增生李斯特菌食品分离株分子型别研究
- Author:
Hua YU
;
Jingcao PAN
;
Haoqiu WANG
;
Guojing SI
;
Tao LIU
;
Xiuqin LOU
;
Wei ZHANG
;
Jie YAN
- Keywords:
Listeria monocytogenes;
multi-locus sequence typing;
pulsed field gel electrophoresis
- From:
Chinese Journal of Zoonoses
2017;33(3):264-270
- CountryChina
- Language:Chinese
-
Abstract:
We determined molecular characteristics of Listeria monocytogenes foodborne isolates in Hangzhou and investigated the characterization of local strains.Multi-locus sequence typing(MLST) and pulsed-field gel electrophoresis(PFGE) were applied to identify molecular types of Listeria monocytogenes isolates.Results showed that a total of 133 strains of 6 serotypes were divided into 19 MLST types including a new type ST767.ST9 and ST121 were the major ST types.There were 33 and 45 PFGE patterns characterized by AscⅠ and ApaⅠ.The molecular types of Listeria monocytogenes strains were widely distributed in Hangzhou.It is indicated that the major clusters were Lineage Ⅰ and Lineage Ⅱ which will cause listeriosis.The contamination of Listeria monocytogenes in food is serious in Hangzhou and the surveillance and management should be strengthened to prevent the food borne diseases.
