Detection and antibiotic sensitivity test of Klebsiella peneumoniae in Ailurus fulgens
10.3969/j.issn.1002-2694.2017.03.015
- VernacularTitle:小熊猫感染肺炎克雷伯氏菌的检查及药敏试验
- Author:
Rui YANG
;
Jifeng WEN
;
Yongping GONG
;
Chengdong WANG
;
Linhua DENG
;
Jie HUANG
;
Lu REN
;
Qigui YAN
- Keywords:
Ailurus fulgens;
K.peneumoniae;
16S rDNA;
LD50;
antibiotic sensitivity test
- From:
Chinese Journal of Zoonoses
2017;33(3):271-275
- CountryChina
- Language:Chinese
-
Abstract:
To confirm the etiology of a dead case for a 6 year-old female Ailurus fulgens,one strain of the predominant bacteria from pathologic tissues(heart,liver,spleen,lung and other samples) of the dead Ailurus fulgens were examined and isolated.The isolate was named R1 and no other bacteria were isolated.The bacterial etiological examination(morphological characteristics,biochemical characteristics and 16S rDNA gene detection)of R1 showed that it was identifed as K.peneumoniae.Artificial infection to mice about R1 was also conducted in this study.R1 had strong pathogenicity to mice and the LD50 is 6.5 × 104 CFU/mL.Moreover,the clinical and pathological features of the dead mice were consistent with that of the Ailurus fulgens.To find effective therapeutic drugs of curing other Ailurus fulgens,antibiotic sensitivity test of R1 was conducted,and the results revealed that R1 was highly sensitive to cefotaxime et al,moderately sensitive to amikacin and resistant to penicillin.These data showed that K.peneumoniae was bacterial pathogen leading to death of the Ailurus fulgens and it had strong resistance to penicillins,macrolides and virginiamycin and it had broad drug resistance spectrum.However,R1 is sensitive to cephalosporins and aminoglycoside antibiotics.
