piggyBac transposon system and its applications in the genemanipulation in mammals
10.3969/j.issn.1005-1678.2017.01.091
- VernacularTitle:piggyBac转座系统及其在哺乳动物基因操作中的应用
- Author:
Qianqian WANG
;
Hui ZHANG
;
Liang XIAO
;
Liming ZHANG
- Keywords:
transposon;
piggyBac (PB);
animal models;
genetic engineering;
gene therapy
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(1):302-305
- CountryChina
- Language:Chinese
-
Abstract:
DNA transposon is a kind of factor that is able to translocate gene in its genome, thus offering an efficient method for permanently modifying the genome of mammals. The piggyBac (PB) transposon system has been proven effective in mammalian genomic engineering, including cancer gene discovery, animal transgenesis, in vivo gene delivery as well as in vitro genetic modification like induced pluripotent stem cells. In addition, piggyBac has many desirable features, such as seamless excision of transposons from the genomic DNA and the potential to target integration events to desired DNA sequences. Therefore, the piggyBac translocation system is an ideal genetic tool in the construction of animal models and gene therapy, and we can anticipate that the piggyBac transposon system will play a more and more important role in biomedical research.
