Study on the Identification of Ferulic Medicinal Plants Based on DNA Barcode ITS2 Sequence
10.6039/j.issn.1001-0408.2017.07.04
- VernacularTitle:基于DNA条形码ITS2序列对阿魏属药用植物的鉴别研究
- Author:
Chong LIU
;
Weijun YANG
;
Jiang HE
;
Bo CHENG
- Keywords:
Ferulic species;
DNA barcode;
ITS2 sequences;
Medicinal materials;
Identification
- From:
China Pharmacy
2017;28(7):878-880
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid,accurate and standardized DNA barcode identification method for the ferulic me-dicinal plants. METHODS:Genomic DNA was extracted from Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M. Shen,ITS2 sequences were amplified and sequenced,analytical similarity search method was conducted,13 species 26 samples gene ITS2 sequences of ferulic medicinal plants in GenBank database were downloaded and comparatively analyzed;interspecies and intraspecies genetic distances were calculated,and phylogenetic tree was developed for cluster analysis. RESULTS:According to calculation,species genetic distances of the 15 species ranged 0.009-0.230,average genetic distance was 0.018;results of clus-ter analysis showed DNA barcode ITS2 sequences can cluster the 32 kinds of ferulic medicinal plants into different classes. CON-CLUSIONS:The established DNA barcode ITS2 sequences identification method can accurately and rapidly identify the genuine and false samples of ferulic species.
