Effects of rAd-p53 Injection on the Proliferation,Apoptosis and Autophagy of Nephroblastoma Cells
10.6039/j.issn.1001-0408.2017.07.07
- VernacularTitle:重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响
- Author:
Zhiyong ZHONG
;
Baojun SHI
;
Hui ZHOU
;
Wenbo WANG
- Keywords:
Nephroblastoma;
rAd-p53 injection;
Cell cycle;
Proliferation;
Apoptosis;
Autophagy
- From:
China Pharmacy
2017;28(7):889-892
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of rAd-p53 injection on the proliferation,apoptosis and autophagy of nephroblas-toma cells. METHODS:Nephroblastoma cells were respectively cultured 20 h with high,medium,low concentration(1×109,1× 108,1×107 VP/mL)of Recombinant human rAd-p53 injection,cells with no injection were the blank control. Cell proliferation,cy-cle,apoptosis and related gene p21,Bax protein expressions were detected,and autophagy gene LC-3,Atg7,Atg12 expressions and number of autophagosomes were also detected. RESULTS:The proliferation inhibition rates of high,medium,low concentra-tion of Recombinant human rAd-p53 injection to nephroblastoma cells were(42.86±3.18)%,(33.64±7.25)%,(16.26±9.07)%;apoptotic rates were (53.85 ± 9.36)%,(37.35 ± 9.64)%,(23.64 ± 10.65)%,respectively. Compared with blank control,cells at period G0/G1 were increased under high,medium,low concentration Recombinant human rAd-p53 injection,effects were relatively obvious under high,medium concentration (P<0.05 or P<0.01);p21,Bax protein and LC-3,Atg7,Atg12 gene expressions were enhanced under high concentration,the number of autophagosomes was increased(P<0.01);Bax protein expression was en-hanced under medium concentration(P<0.05),the other indicators had no obvious changed(P>0.05). CONCLUSIONS:Recom-binant human rAd-p53 injection can inhibit the cell proliferation of nephroblastoma,and induce its apoptosis and autophagy.
