Effects of Yunnan BaiYao on the Expressions of c-fos and C-jan mRNA in Periodontal Ligament Cells in Vitro
- VernacularTitle:云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响
- Author:
Wen CHU
;
Bing WANG
;
Yinuo ZHANG
;
Lixia HAO
;
Wan WANG
;
Yanxu GUO
;
Zhilu WANG
- Keywords:
Yunnna BaiYao;
Human periodontal ligament cells;
C-fos;
C-jun;
Proliferation and differentiation
- From:
Journal of Kunming Medical University
2016;37(9):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Yunnan BaiYao on the expressions of c-fos and c-jun mRNA in HPDLFs and explore the potential mechanism that Yunnan BaiYao promotes the proliferation and differentiation of HPDLFs.Methods The HPDLFs tissue was obtained from the extracted healthy premolar.The HPDLFs used underwent four to six passages.Cells were divided into untreated group,positive control group and treated group.In treated group,HPDLFs were co-cultured with Yunnan BaiYao solution for 4h with gradient concentration.The expressions of c-fos and c-jun mRNA were determined by RT-PCR.Results The results showed that the expressions of c-fos and c-jun mRNA were up-regulated significantly in treated group compared with control group (P＜0.05) Conclusion Yunnna BaiYao can upregulate the expressions of c-los and C-jun mRNA in HPDLFs.Through upregulating the expressions of c-fos and c-jun mRNA in hPDLs,Yunnan BaiYao can promote proliferation and differentiation of ossification of HPDLFs to induce bone formation.
