The Gene Expression Change of Inflammatory Factors TNF-α, IL-6 and IL-1β in Cerebral Ischemia-Reperfusion Rats
- VernacularTitle:脑缺血-再灌注损伤大鼠脑组织中TNF-α,IL-6和IL-1β的表达
- Author:
Junjie LI
;
Haiyan JIANG
;
Jianlin SHAO
- Keywords:
Cerebral ischemia;
Reperfusion injury;
Quantitative Real-Time PCR;
Inflammatory factors
- From:
Journal of Kunming Medical University
2016;37(9):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene expression change of TNF-α,IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury.Methods A total of 24 adult male SD rats were randomly divided into four groups:sham group and 3 groups with brain ischemia reperfusion of 3h,6h and 12h.Real-Time PCR was used to analyze the gene expression ofTNF-α,IL-6 and IL-1 β at 3h,6h,and 12h after reperfusion.Results In the sham group,the mRNA expression levels of TNF-α,IL-6 and IL-1 β in were low,but increased immediately after brain ischemia injury and decreased gradually thereafter.The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak (P ＜0.01) then significantly decreased at 12h after reperfusion.The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h (P＜0.01),and significantly decreased at 12h compared with 6h (P＜0.01).The gene expression of IL-1 β mRNA at 3h after reperfusion was significantly increased,peaked at 6h (P＜0.01) and significantly decreased at 12h (P ＜0.01).Conclusion The gene expression levels of TNF-α,IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak,which suggested that the gene expression change of TNF-α,IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury.
