The role of spo0A gene in growth and sporulation of Clostridium difficile
10.16718/j.1009-7708.2017.01.006
- VernacularTitle:spo0A基因在艰难梭菌生长及芽孢形成中的作用
- Author:
Xiaolei SONG
;
Fenfen ZHOU
;
Shi WU
;
Qiong GAO
;
Haihui HUANG
;
Yijian CHEN
- Keywords:
Clostridium difficile;
sporulation;
spo0Agene;
growth curve
- From:
Chinese Journal of Infection and Chemotherapy
2017;17(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of spo0A gene in growth and sporulation of Clostridium difficile clinical isolates. Methods ClosTron gene knock-out system was used to knock out the spo0A gene of C. difficile strain C25. Bacterial growth curve was plotted by measuring D600 with spectrophotometer in different phases of bacterial growth. Malachite green staining technique was used to count the number of vegetative cells and spores under optical microscope. The sporulation rate was calculated. Results The spo0A mutant and its C25 parental strain showed similar patterns of growth. However, after knock-out of spo0A gene, an asporogenous phenotype was built, while the parental strain could produce spores as usual.Conclusions The spo0A gene plays a key role in sporulation but not growth of C. difficile strain.
