Effect of CCK-8 on expression of MMPs/TIMP-1 in TNF-α-induced RSC-364
10.3969/j.issn.1001-1978.2017.04.023
- VernacularTitle:CCK-8对TNF-α诱导大鼠RSC-364细胞MMPs/TIMP-1的影响
- Author:
Jinrong XU
;
Bin CONG
;
Shujin LI
;
Yuhuai JIN
;
Zhansheng ZHAO
- Keywords:
cholecystokinin-octapeptide;
TNF-α;
arthritis;
fibroblast-like synoviocytes;
matrix metalloproteinases;
tissue inhibitors of metalloproteinases
- From:
Chinese Pharmacological Bulletin
2017;33(4):567-571
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the influence of CCK-8 on expression of MMPs/TIMP-1 in TNF-α-induced rat fibroblast-like synovial cell line RSC-364.Methods The secretion levels of MMP-1, MMP-3, MMP-9 and TIMP-1 were determined using ELISA;MMP-3 and MMP-9 mRNA expressions were detected by RT-PCR.Results MMP-3 and MMP-9 could not be examined in RSC-364 incubated with CCK-8 and unstimulated RSC-364, which was able to product a little MMP-1, TIMP-1 and express even less MMP-3,-9 mRNA.CCK-8 inhibited the increase in MMP-1, MMP-3, MMP-9 secretion and MMP-3,-9 mRNA expression in TNF-α-induced RSC-364.TIMP-1 production was also increased in TNF-α-induced RSC-364.CCK-8 had no effect on TIMP-1 production in TNF-α-induced RSC-364, but was able to reduce the ratios of MMP-1, MMP-3, MMP-9 to TIMP-1.Conclusion The inhibitory effect of CCK-8 on MMPs activity may be related to the decrease of MMPs mRNA expression, MMPs secretion and the ratios of MMPs to TIMP-1 in TNF-α-induced RSC-364, which indicates that CCK-8 might be a possible regulator in the pathogenesis of rheumatoid arthritis.
