Effect of Tongxinluo on polarization of macrophages
10.3969/j.issn.1001-1978.2017.04.025
- VernacularTitle:通心络对巨噬细胞极化的影响
- Author:
Hongrong LI
;
Liping CHANG
;
Yujin LIU
;
Cong WEI
;
Junqing LIANG
;
Zhenhua JIA
- Keywords:
atherosclerosis;
tongxinluo;
macrophage;
polarization;
notch;
inflammation
- From:
Chinese Pharmacological Bulletin
2017;33(4):577-580
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of Tongxinluo on the polarization of macrophages to type M2 and M1, and on Notch signaling pathway.Methods THP-1 was respectively induced by PMA and IL-4 or LPS to M2 and M1 type polarized macrophages, and treated by Tongxinluo.The expressions of marker molecules IL-10, TNF-α, IL-6 and protein of Notch signaling pathway were detected.Results The level of IL-10 increased in M2 group and there was no statistical difference on the level of IL-6 and TNF-α;the level of IL-10 decreased and IL-6, TNF-α increased in M1 group;the level of IL-10 increased and IL-6,TNF-α decreased in TXL group.There was no statistical difference in the expression of active Notch-1, DLL4 and Hes-1 between control group and M2 group;the expression of active Notch-1, DLL4 and HES-1 increased significantly in M1 group;the expression of active Notch-1, DLL4 and HES-1 decreased significantly in TXL group;there was no significant difference in the expression of Notch-1 protein in each group.Conclusion The polarization of macrophages to M1 type can be inhibited by Tongxinluo, and its mechanism may be related to the inhibition of activation of Notch-1 signaling pathway.
