Improvement of Cardiomyocyte Apoptosis Induced by Hydrogen Peroxide via the Intervention of GLP-1 Signal Pathway
10.11969/j.issn.1673-548X.2017.03.025
- VernacularTitle:干预GLP-1通路保护过氧化氢诱导心肌细胞凋亡的研究
- Author:
Shumei ZHAO
;
Qian ZHANG
;
Chunyan GUO
- Keywords:
GLP-1 signal pathway;
Oxidative stress;
Cardiomyocyte apoptosis;
PI3K/AKT
- From:
Journal of Medical Research
2017;46(3):98-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Exendin-4 on the apoptosis of cardiomyocyte induced by H2O2,and approach the relationship between GLP-1 signal pathway and the injury of cardiomyocyte.Methods Cardiomyocytes were isolated and cultured,and were divided into 5 groups.Intercelluar ROS (reactive oxygen species) was measured,and cell apoptosis rate was evaluated by Flow cytometry in different groups.Also expressions of apoptosis-associated proteins (caspase-3) and PI3 K/AKT were evaluated by western blot.Results Compared with H2O2 group,Ex-4 co-incubation decreased the production of intercelluar ROS levels,also improved the cardiomyocyte apoptosis.At the same time,Ex-4 resulted in the alterations in expressions of the caspase-3 and p-AKT/AKT proteins.However,these effects of Exendin-4 were counteracted significantly by the co-incubation of LY294002.Conclusion The interventions of GLP-1 signal pathway can improve cardiomyocyte apoptosis induced by H2O2 incubation,and the mechanisms might partly attribute to the PI3K/AKT system.
