Changes of liver fibrosis-related miRNAs induced by soluble egg antigen of Schistosoma japonicum
10.16250/j.32.1374.2017010
- VernacularTitle:日本血吸虫可溶性虫卵抗原诱导肝纤维化相关miRNA的变化
- Author:
Huan WANG
;
Yajing LU
;
Yanru GAO
;
Shuhong WANG
;
Rui ZHOU
;
Huifen DONG
- Keywords:
Schistosoma japonicum;
Liver fibrosis;
miRNA;
Soluble egg antigen(SEA);
Hepatocytes
- From:
Chinese Journal of Schistosomiasis Control
2017;29(2):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miRNA associated with hepatic fibrosis induced by Schistosoma ja-ponicum soluble egg antigen stimulation in mouse hepatocytes(AML12),so as to lay the foundation for clarifying the mecha-nism of schistosome infection leading to hepatic fibrosis. Methods The expressions of miR-122,miR-182,miR-23b,miR-27b and KSRP in AML12 cells treated with SEA were measured by q-PCR. KSRP protein in cell lyses was measured by Western blotting. AML12 cells were transfected with miR-27b precursor or anti-miR-27b for 24 h,then q-PCR was adopted to determine KSRP mRNA,and KSRP protein was detected by Western blotting. Results The expressions of miR-182,miR-23b and miR-27b were decreased and miR-122 was increased in AML12 cells following SEA treatment(all P<0.05). An increase of mRNA and protein of KSRP expression was also observed in AML12 cells after SEA stimulation(both P<0.05). In addition,KSRP mRNA expression was not changed significantly in AML12 cells transfected with anti-miR-27b or miR-27b precursor,and miR-27b precursor reduced KSRP protein expression as compared with the control. In contrast,the expression of KSRP protein was increased in the anti-miR-27b group and decreased in the miR-27b precursor group. Conclusions After the stimulation of SEA,the expressions of a variety of liver fibrosis-related miRNAs and KSRP change in murine hepatocytes,including miR-27b. And miR-27b can regulate the expression of KSRP. These findings might lay a foundation for further study on the molecular mechanism of fibrosis induced by schistosome infection.
