Effect of allopurinol on serum level of uric acidand intestinal expression of glucose transportersin rats with fructose-induced hyperuricemia
10.3969/j.issn.1001-1978.2017.04.006
- VernacularTitle:别嘌醇改善果糖诱导的大鼠高尿酸血症与调节肠道葡萄糖转运子表达的关系研究
- Author:
Gang CHEN
;
Ping JIA
- Keywords:
allopurinol;
fructose;
uric acid;
hyperuricemia;
glucose transporter 2;
glucose transporter 5
- From:
Chinese Pharmacological Bulletin
2017;33(4):469-474
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of allopurinol and benzbromarone on serum level of uric acid, hepatic xanthine oxidase(XOD) activity and intestinal expression of glucose transporter(GLUT) 2 and 5 in rats with fructose-induced hyperuricemia.Methods Wistar rats were fed with 10% fructose in drinking water for consecutive 8 weeks to induce HUA.Treatment with 5 mg·kg-1 allopurinol or 10 mg·kg-1 benzbromarone were intragastricly administered from 5~8 weeks.Serum level of uric acid and XOD activity in liver were tested.Expression of GLUT2 and GLUT5 in intestine was analyzed by immunohistochemistry staining and Western blot.Results Treatment with allopurinol or benzbromarone significantly decreased the serum level of uric acid in fructose-induced hyperuricemic rats.At the same time, allopurinol treatment significantly reduced the XOD activity in liver and GLUT5 expression in intestine.Nevertheless, benzbromarone treatment did not show inhibitory effect on hepatic XOD activity and intestinal GLUT5 expression.In addition, neither allopurinol nor benzbromarone showed inhibitory effect on GLUT2 expression in intestine.Conclusions Allopurinol decreases serum level of uric acid in fructose-induced hyperuricemic rats.The mechanism is related to reducing XOD-mediated uric acid production in liver, and decreasing GLUT5-mediated fructose absorption in intestine.
