Total flavonoids from arachniodes exilispromotes osteogenic differentiation of hUCMSCs

VernacularTitle:刺头复叶耳蕨总黄酮促进脐带间充质干细胞成骨分化
Author: Chen YAN ; Changchang YIN ; Ziyao WANG ; Ming YIN
Keywords: TFAE; hUCMSCs; proliferation; Col1a1; OPN; osteogenic differentiation
From: Chinese Pharmacological Bulletin 2017;33(4):557-562
CountryChina
Language:Chinese
Abstract: Aim To study the role of the total flavonoids from arachniodes exilis(TFAE) in osteogenic differentiation of human umbilical cord mesenchymal stem cells(hUCMSCs).Methods hUCMSCs were isolated and cultured by tissue explants adherent method, hUCMSCs at passage 3 were used to do the experiment, hUCMSCs were treated by different concentrations of TFAE, and cellular viability was measured by CCK 8 assay;alkaline phosphatase(ALP) activity was tested by AMP method;calcium nodule formation was detected by alizarin red staining;expression level of osteogenesis-related gene type I collagen enzyme a1(collagen type Ia1, Col1a1), osteopontin(OPN), Runx2, Osterix(Osx) mRNA was measured by RT-PCR;expression level of Col1a1,OPN protein was measured by Western blot.Results Certain concentration of TFAE(1 mg·L-1, 5 mg·L-1) promoted cellular proliferation, calcium nodules were more, ALP activity was enhanced;expression level of osteogenesis-related gene Col1a1, OPN, Runx2, OsxmRNA and Col1a1, OPN protein was up-regulated.Conculsion A certain concentration of TFAE promotes proliferation and osteogenic differentiation of hUCMSCs.