Characteristics and immuno-modulatory effects of Enterococcus faecium JS1-8 isolated from Kimchi.
10.12729/jbr.2014.15.4.182
- Author:
Su Bin AHN
1
;
Ho Eun PARK
;
Sang Myeong LEE
;
So Young KIM
;
Wan Kyu LEE
Author Information
1. College of Veterinary Medicine, Chungbuk National University, Cheongju 362-763, Korea. wklee@cbu.ac.kr
- Publication Type:Original Article
- Keywords:
Enterococcus faecium;
bacteriocin;
probiotics;
immuno-modulatory effect;
Kimchi
- MeSH:
Bacteria;
Bile;
Caco-2 Cells;
Cytokines;
Enterococcus;
Enterococcus faecium*;
Hot Temperature;
Humans;
Hydrogen-Ion Concentration;
Interleukin-1beta;
Lactic Acid;
Lactobacillus;
Lactobacillus rhamnosus;
Listeria monocytogenes;
NF-kappa B;
Nitric Oxide;
Probiotics;
Salmonella enteritidis;
Staphylococcus aureus;
Tumor Necrosis Factor-alpha
- From:Journal of Biomedical Research
2014;15(4):182-188
- CountryRepublic of Korea
- Language:English
-
Abstract:
Characteristics and immuno-modulatory effects of Enterococcus (E.) faecium JS1-8 isolated from Kimchi were investigated for potential probiotic use. We measured their acid, bile, and heat tolerances, adhesion properties in Caco-2 cells, antimicrobial activity against pathogenic bacteria, and bacteriocin-like substance-producing activity. Immuno-modulatory effects of E. faecium JS1-8 were measured by determination of nitric oxide (NO), nuclear factor-kappaB (NF-kappaB), interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) production in RAW 264.7 cells or RAW BLUE cells. JS1-8 survived at pH 2.0 for 2 hr and showed tolerance to 0.3% oxgall bile salt, and it survived after exposure for 5 min at 80degreesC. JS1-8 showed high antimicrobial inhibition zones to Staphylococcus aureus (460 mm), Listeria monocytogenes (310 mm), Salmonella enteritidis (280 mm), and E. coli (150 mm). Bacteriocin-like substance produced by JS1-8 showed a broad spectrum of activity against Listeria monocytogenes KCCM 40307 and Lactobacillus sake KCCM 40264. Low concentration (1 x 107 CFU/mL) of heat-killed E. faecium JS1-8 induced statistically higher production of NO than Lactobacillus rhamnosus GG (LGG), which is a well-known immuno-modulatory lactic acid bacteria. Low and high (5 x 107 CFU/mL) concentrations of JS1-8 induced statistically higher production of NF-kappaB than that produced by LGG. We also found that JS1-8 increased production of pro-inflammatory cytokines such as IL-1beta and TNF-alpha in a concentration-dependent manner. As a result, E. faecium JSI-8 could be used as a useful probiotic for controlling pathogens and enhancing host immune responses.
