Uncertainty Evaluation for the Determination of Pseudo-ginsenoside GQ in Human Plasma by HPLC-MS/MS
10.3870/j.issn.1004-0781.2017.03.007
- VernacularTitle:HPLC-MS/MS法测定人血浆伪人参皂苷GQ浓度的不确定度评定
- Author:
Jiping HUO
;
Shenghui MEI
;
Zhigang ZHAO
;
Hongyun WANG
;
Pei HU
;
Pingya LI
;
Jinping LIU
;
Ji JIANG
- Keywords:
Pseudo-ginsenoside GQ;
HPLC-MS/MS;
Uncertainty
- From:
Herald of Medicine
2017;36(3):262-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the uncertainty of the pseudo-ginsenoside GQ (PGQ) concentration in human plasma by HPLC-MS/MS.Methods The whole process of PGQ determination by HPLC-MS/MS in human plasma was evaluated and the uncertainty caused by repeatability,weighing,standard solution preparation,biological sample preparation,extraction recovery process,recovery,instrument precision and calibration curve fitting were evaluated,respectively.The combined and expanded uncertainty values were both calculated.Results The expanded uncertainty values for low (15.16 ng·mL-1),medium (2 516.67 ng·mL-1) and high (3 902.00 ng·mL-1) levels of PGQ were 1.39,177.74 and 262.69 ng·mL-1,respectively (P =95 ％,k =2).Conclusion The uncertainty of the PGQ determination in human plasma by HPLC-MS/MS is mainly caused by recovery,repeatabihty and sample preparation at low concentration,by sample preparation and recovery at medium and high concentration.
