Protective effect of Baicalin on experimental autoimmune encephalomyelitis in mice

VernacularTitle:黄岑苷对实验性自身免疫性脑脊髓炎小鼠的影响
Author: Yingguo REN ; Baochao ZHANG ; Dongpei JIA ; Ke HU
Keywords: Baicalin; Experimental autoimmune encephalomyelitis; Cell apoptosis; Mitochondria; Multiple sclerosis
From: Chinese Journal of Comparative Medicine 2017;27(3):52-56
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of different concentrations of baicalin on the mouse model of experimental autoimmune encephalomyelitis (EAE) and to explore its mechanisms.Methods A mouse model of EAE was established with MOG33-55 peptide and bacillus Calmette-Guerin (BGG) vaccine with complete Freund adjuvant (CFA).At the third day after immunization, high and low doses of baicalin were administered to the mice intragastrically once a day for 20 days.The neurological function of mice was evaluated.TUNEL staining was used to detect apoptosis in the spinal cord tissue.The level of ATP in spinal cord tissue was detected by an ATP determination kit.Furthermore, the protein expressions of Bax, Bcl-2, cleaved cas-3 and cleaved cas-9 were detected by western blot, respectively.Results Baicalin improved the neurological function and delayed the onset time in EAE mice.