Effects of 17β-estradiol on stromal cell-derived factor-1 expression after retinal ischemia-reperfusion injury
10.13389/j.cnki.rao.2017.0055
- VernacularTitle:雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响
- Author:
Yeqing WANG
;
Xiaomei ZHANG
;
Li DONG
;
Huanqi SHI
;
Wenjiao BI
;
Wenwen HOU
- Keywords:
retina;
ischemia-reperfusion;
17β-estradiol;
stromal cell-derived factor-1
- From:
Recent Advances in Ophthalmology
2017;37(3):215-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of stromal cell-derived factor-1 (SDF-1) in a rat model of retinal ischemia-reperfusion injury (RIRI),and investigate the protective effect of 17β-Estradiol (E2) on RIRI and explore the mechanism.Methods The RIRI model was established in Sprague-Dawley rats by increasing the intraocular pressure.Relative expression levels of SDF-1 mRNA and protein in the retina at 6 hours,12 hours and 24 hours following reperfusion was determined by RT-PCR and Western blot,respectively.E2 was administered to investigate the effects of estrogen on SDF-1 expression,and the estrogen receptor antagonist ICI 182-780 was administered to investigate the effect of estrogen receptor on the expression of SDF-1.Results SDF-1 expression in RIRI 6 hours group,12 hours group and 24 hours group was increased compared with normal control group (all P ＜ 0.05),with maximum expression at RIRI 12 hours group.As expected,pretreatment of RIRI rats with E2 had a protection on RIRI retina;SDF-1 expression was increased in RIRI + E2 group compared with IR control group and RIRI + vehicle group (all P ＜ 0.05).RIRI + E2 + ICI 182-780 group could decrease SDF-1 expression compared with RIRI + E2 group(all P ＜ 0.05).Conclusion E2 offers protection against RIRI by inducing an up-regulation in SDF-1 expression through activation of the estrogen receptor.
