Influence of fermented red ginseng extract in proliferation of rat glomerular mesangial cells and extracellular matrix degradation under high sugar stimulation
10.13481/j.1671-587x.20170207
- VernacularTitle:红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响
- Author:
Meng QU
;
Jiajing DONG
;
Rui JIANG
;
Jichun CUI
;
Kunyang SUN
;
Zhiheng DONG
;
Liwei SUN
- Keywords:
fermented red ginseng extract;
diabetic nephropathy;
glomerular mesangial cells;
cell proliferation;
matrix metalloproteinases
- From:
Journal of Jilin University(Medicine Edition)
2017;43(2):245-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of fermented red ginseng extract (FRGE) in the proliferation of rat glomerular mesangial cells (GMCs) and the degradation of extracellular matrix(ECM)under high sugar stimulation, and to clarify the prevention and treatment effects of FRGE on diabetic nephropathy (DN) and the possible mechanism.Methods:The rat GMCs were cultured and divided into normal concentration of D-glucose (NG) group, high concentration of D-glucose (HG) group and high concentration of D-glucose plus different concentrations (3.75, 7.50, 15.00 mg·L-1) of FRGE groups. The proliferation rates of rat GMCs were detected with MTT,and the type Ⅳcollagen(Col Ⅳ) levels in supernatants of the GMCs were detected by ELISA. The protein expressions levels of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) were detected with Western blotting m ethod.Results:Compared with NG group, the proliferation rate of GMCs in HG group was increased(P<0.01), the Col Ⅳ level was increased(P<0.01),the MMP-2 expression level was decreased, and the TIMP-2 expression level was up-regulated(P<0.01).Compared with HG group, the proliferation rates of GMCs in various FRGE groups were decreased(P<0.01), the Col Ⅳ levels were decreased(P<0.01),the expression levels of TIMP-2 were reduced(P<0.01),and the expression levels of MMP-2 were increased(P<0.01).Conclusion:FRGE can inhibit the proliferation of rat GMCs induced by high sugar and promote the ECM degradation to delay the occurrence and development of DN.
