Effects of Leukocyte-and Platelet-rich Fibrin on Tendon-bone Healing of Rabbits' Extra-articular Bone Tunnel in the Early Period
- VernacularTitle:自体外周血富白细胞-血小板纤维蛋白对兔关节外骨道内移植肌腱腱骨早期愈合的影响
- Author:
Yijun LIU
;
Bin XU
;
Jun TU
;
Gang YU
- Keywords:
leukocyte-and platelet-rich fibrin;
extra-articular;
tendon bone healing
- From:Chinese Journal of Sports Medicine
2017;36(3):207-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the early effect of leukocyte-and platelet-rich fibrin (L-PRF)on tendon-bone healing in rabbits' extra-articular bone tunnel.Methods Thirty healthy New Zealand rabbits were randomly divided into an experimental group and a control group,each of 15.The semitendinosus tendon harvested from the hind leg of each rabbit was prepared as free autografts.Each autograft was implanted into the bone tunnel created at ipsilateral proximal tibial metaphysis.The experimental group was added with autologous peripheral blood-derived L-PRF,while the control group did not add anything.Speciments from each group were harvested at 4,8 and 12 weeks after operation.The morphological changes were observed and evaluated at each time point.The fibroblasts on the tendon-bone interface were counted and analyzed under the high magnification microscope.Results The number of fibroblasts between the tendon and bone in the experimental group was significantly higher than that of the control group at each time point (P＜0.05).And the connection between tendon and bone was closer and the collagen fibers were more regular in the experimental group than the control group at each time point.Sharpey's-like fibers which marked the early healing of the tendon to bone,were observed at 4th week in the experimental group,while they were observed at 8th week in the control group.As the healing time extended,Sharpey's like fibers continue to increase in both groups and significant differences were observed in the histological morphology (Buark grades)between the two groups (P＜0.05).Conclusion Autologous peripheral blood-derived L-PRF can promote early healing of autologous tendon to bone in the extra-articular bone tunnel of model rabbit.
