Light-inducible CRISPR/Cas9 system for control of gene expression:research advances
10.13220/j.cnki.jipr.2017.03.001
- VernacularTitle:光诱导CRISPR/Cas9系统在基因表达调控中的研究进展
- Author:
Liting LAN
;
Xiaoli WEI
;
Haitao YAN
;
Ruibin SU
- Keywords:
light;
CRISPR/Cas9;
dCas9;
transcription activation;
transcription inhibition
- From:
Journal of International Pharmaceutical Research
2017;44(3):215-219
- CountryChina
- Language:Chinese
-
Abstract:
Cas9 is a RNA-guided double stranded DNA nuclease that participates in the CRISPR/Cas9 system. Wide-type Cas9 directly silences the expression of target gene by gene splicing. The engineered dCas9 protein with the mutation at D10A and H840A lacks the Cas9' s endonuclease function but keeps its DNA binding activity. dCas9 can activate special genes by fusing with transcription activator. Meanwhile,it can inhibit the gene transcription by directly binding to the target gene and stop gene transcrip?tion. Combination of light sensitive structures and CRISPR can produce light-inducible CRISPR/Cas9 system for control of gene expres?sion. This system is able to activate or inhibit gene expression via the use of controlling blue light(470 nm). In this review,we mainly discuss the development of the light inducible CRISPR/Cas9 system as well as its application in the control of gene expression.
