Effect of EMS1-siRNA on the proliferation and migrationof human gastric cancer cell line MGC803

VernacularTitle:RNA干扰EMS1基因表达对人胃癌MGC803细胞增殖和迁移的影响
Author: Suping HOU ; Xiyan TONG ; Hailong XIE
Keywords: small interference RNA; EMS1 gene; proliferation; migration
From: Chinese Journal of Clinical and Experimental Pathology 2016;32(12):1317-1322
CountryChina
Language:Chinese
Abstract: Purpose To explore the effect of EMS1-siRNA on the growth,invasion and migration of human gastric cancer cell line MGC803.Methods It used the Colony formation assay to determine the abilities of proliferation,and flow cytometry analysis to asses cell cycle distribution and apoptosis,transwell invasion and migration experiment to determine the ability of cell invasion and migration after knockdown the expression of EMS1 in MGC803.Results These results suggested that EMS1 gene down-regulated have no affect on cell cycle and cell apoptosis,but the ability of colony formation depressed and migration lowerd obviously (P ＜ 0.05).Conclusion The results shows EMS1 gene is related to proliferation and migration of tumor.