ID3, TCF3 and MYC genes mutation analyses in Chinese Burkitt lymphoma
10.13315/j.cnki.cjcep.2016.12.006
- VernacularTitle:中国人Burkitt淋巴瘤中ID3、TCF3及MYC基因突变分析
- Author:
Jie WEI
;
Linni FAN
;
Xia LI
;
Mingyang LI
;
Yixiong LIU
;
Danhui ZHAO
;
Qingguo YAN
;
Shuangping GUO
;
Xiaochu YAN
;
Zhe WANG
- Keywords:
lymphoma;
Burkitt lymphoma;
ID3;
TCF3;
MYC;
Sanger sequencing
- From:
Chinese Journal of Clinical and Experimental Pathology
2016;32(12):1343-1347
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the mutations of ID3,TCF3 and MYC genes in Chinese Burkitt lymphoma and discuss their significance.Methods Total DNA was extracted from tumor tissues of 32 patients with Burkitt lymphoma,then the DNA was amplified by polymerase chain reaction (PCR),and the products of PCR were sequenced directly with Sanger sequencing methods.Results The mutation rates of ID3 and TCF3 genes were 35.5％ (11/31) and 18.8％ (6/32) respectively.The mutation rate of MYC was 50％.The mutation rates of MYC exon 1,MYC exon 2 and MYC exon 3 were 3.3％ (1/30),50％ (15/30) and 7.7％ (2/26) respectively.Conclusion Recurrent mutations of the ID3,TCF3 and MYC genes in Chinese Burkitt lymphoma were identified by Sanger sequencing.For TCF3 gene,a novel mutation c.2202G ＞ C p.L569V was found in three cases.In two cases,a novel mutation of c.1070A ＞G p.G182D was found in MYC gene.
