The Microtox-Based Fast Test of Comprehensive Toxicity of Safflower Injection
10.11842/wst.2016.11.015
- VernacularTitle:Microtox(微毒)技术应用于红花注射液综合毒性检测
- Author:
Limin LUO
;
Liangchun YAH
;
Tianxi WEI
;
Hua HUA
;
Yongjun ZHU
;
Junning ZHAO
- Keywords:
Microtox technology;
luminous bacteria;
safflower injection;
comprehensive toxicity
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(11):1935-1941
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at exploring a new method--microtox technology to evaluate the comprehensive toxicity of safflower injections from different drug manufacturers.In the experiment,we investigated the characteristic parameters of vibrio fischeri (CS234) under different conditions,and then locked the optimum parameters for safflower injection assay:recovery liquid volume 0.9 mL in freeze-dried vial,50 μL bacteria suspension in each sample,and the detection time of 10 mins after adding the bacteria suspension.Under this optimum detection condition,we found that the EC50 values were 3.36％,5.58％ and 4.33％,being significantly different between 3 representative drug manufacturers (P ＜ 0.05).In conclusion,it was implied that the biological detection standards of safflower injections need improving,while the microtox-based fast test of the comprehensive toxicity of safflower injection showed a favorable and prospective application to controlling the product qualities of different manufacturers.
