Regulation of the Circadian Gene CLOCK Expression by KCl Depolarization in B35 Rat Neuroblastoma Cells.
- Author:
Won Je JEON
1
;
Se Hyun KIM
;
Myoung Suk SEO
;
Ung Gu KANG
;
Yong Sik KIM
;
Yong Min AHN
Author Information
1. Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Expression of CLOCK;
Circadian clocks;
Depolarization
- MeSH:
Animals;
Circadian Clocks;
Cycloheximide;
Dactinomycin;
Immunoblotting;
Incubators;
Neuroblastoma*;
Rats*
- From:Journal of Korean Neuropsychiatric Association
2006;45(1):21-27
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate the effects of KCl on regulation of circadian gene CLOCK expression, we observed whether induction of CLOCK is influenced by KCl depolarization in B35 rat neuroblastoma cells. METHODS: B35 rat neuroblastoma cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin-streptomycin in a 37 degrees C humidified incubator with 5% CO2. Inhibitors including cycloheximide and actinomycin D were pretreated 1 hour before treatment with 50mM KCl. Immunoblotting with anti-CLOCK antibody was done. RESULTS: CLCOK is induced by 50 mM KCl in B35 Rat Neuroblastoma cells, and a maximal induction in CLOCK level reached peak at 8 to 20 hours. The pretreatment of cycloheximide and actinomycin D prevented the induction of CLOCK by 50 mM KCl. CONCLUSION: We suggest that KCl depolarization may play critical roles in several aspects of the circadian gene CLOCK expression.
