Multiplex PCR and detection of three genes of Helicobacter pylori
- Author:
Nguyen Thi Thanh Loi
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori
- MeSH:
Helicobacter pylori;
diagnosis;
Genes
- From:Journal of Medical Research
2003;0(2):1-4
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Bacterium Helicobacter pylori is the causative agent of many gastric disorders. That is why, its correct detection is necessary. However, monitoring the bacteria by amplifying only one gene has used to give false-negative results due to the biodiversity of the bacteria. The conditions for Multiplex PCR amplifying three virulent genes of the bacteria such as cagA., ureaseB and Hp1125 were optimized. It is recommended to detect Helicobacter pylori in clinical setting in Vietnam.
