Antiplatelet aggregation of naringenin via inhibiting PI3K/Akt signaling in vitro
10.3969/j.issn.1000-4718.2017.03.022
- VernacularTitle:柚皮素通过PI3K/Akt通路拮抗血小板聚集的体外研究
- Author:
Manting HUANG
;
Huanlin WU
;
Danping XU
- Keywords:
Naringenin;
Platelet aggregation;
Adenosine diphosphate;
PI3K/Akt signaling pathway
- From:
Chinese Journal of Pathophysiology
2017;33(3):517-522
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the antiplatelet aggregation of naringenin and its possible mechanism . METHODS:The effects of naringenin at different concentrations on adenosine diphosphate ( ADP)-induced platelet aggre-gation and platelet spreading on immobilized fibrinogen were detected by aggregational detector and observed under fluores -cence microscope, respectively.The expression of phosphoinositide 3-kinase (PI3K) and the phosphorylation of Akt were analyzed by Western blot .RESULTS:Naringenin significantly inhibited ADP-induced platelet aggregation in a dose-de-pendent manner in vitro and in vivo, and inhibited platelet ' s spreading on immobilized fibrinogen in vitro.Further studies indicated that naringenin inhibited platelet activation accompanied by attenuating not only the activation of PI 3K, but also the phosphorylation of Akt .Moreover, naringenin combined with LY294002 additively inhibited the ADP-induced platelet aggregation.CONCLUSION:Naringenin may inhibit platelet activation by regulation of PI 3K/Akt signaling pathway.
