Optimization of Extraction Process for Polysaccharide from Fermented Cordyceps Sinensis Powder by Response Surface Methodology
10.13359/j.cnki.gzxbtcm.2017.02.024
- VernacularTitle:响应面分析法优化发酵虫草菌粉多糖的提取工艺
- Author:
Heyun NIE
;
Qinghua ZHANG
;
Dongmei YAN
;
Lihua CHEN
;
Yongmei GUAN
;
Ming YANG
- Keywords:
polysaccharide from fermented Cordyceps Sinensis powder;
response surface analysis;
extraction rate;
Box-Behnken design
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(2):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction process for the polysaccharide from fermented Cordyceps Sinensis powder with response surface methodology.Methods We selected the factors and levels on the basis of single factor experiment,and then designed the experiment with 3 factors and 3 levels based on the principle of Box-Behnken's design.Results The effect of the factors of extraction temperature,time and solid-liquid ratio on extraction ratio was in decreasing sequence.The optimal extraction technology obtained through the classical analysis was as follows:extraction temperature at 95 ℃,the ratio of solid to liquid being 1 ∶ 21,and extraction for 73 min.Under this condition the theoretical extraction rate was 4.31％ and the actual extraction rate was (4.20 ± 0.1)％.Conclusion The obtained values agree with the predicted values of the mathematic models,and the Box-Behnken experimental design is suitable for optimizing the extraction of the polysaccharide from fermented Cordyceps Sinensis powder.
