Optimization of Sulfated Technology of Rhizoma Smilacis Glabrae Polysaccharides by Orthogonal Design and Observation of Anti-tumor Activity of Modified Products
10.13359/j.cnki.gzxbtcm.2017.02.025
- VernacularTitle:正交实验优选土茯苓多糖的硫酸化工艺研究及修饰产物抗肿瘤活性测定
- Author:
Jun QIN
;
Guanghai DENG
;
Mingchao LUO
;
Wei ZHU
- Keywords:
Rhizoma Smilacis Glabrae polysaccharides;
orthogonal design: sulfating modification;
anti-tumor activity
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(2):254-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the sulfated modification conditions for Rhizoma Smilacis Glabrae polysaccharides (RSGP),and to investigate the possibility of enhancing the activity of RSGP after sulfating modification.Methods RSGP was modified by cholorosulfonic acid-pyridine.With the yield,carbohydrate content and sulphate substitution degree as the observation indexes,L9 (34)orthogonal design was used to optimize reaction time,reaction temperature and reagent ratio.The degree of sulphate substitution was determined by barium chloride turbitimetry,and the carbohydrate content was detected by sulfuric acid-phenol method.Then the structures of the sulfated modified products were analyzed by infrared radiation (IR) spectrum.Methyl thiazolyl tetrazolim(MTT)assay was used to determine the anti-tumor activity of sulfated RSGP on HepG2 and MCF-7 cell lines.Results The optimized modification conditions of RSGP were sulfating RSGP with cholorosulfonic acid-pyridine in the ratio of 1:6 for 4.5 hours at 60 ℃.The MTT assay results showed that the sulfated RSGP could inhibit the growth and proliferation of human liver cancer HepG2 cell line and human mammary cancer MCF-7 cell line in concentrationdepending manner.Conclusion It is feasible for sulfating modification of RSGP with cholorosulfonic acid-pyridine,and sulfating modification can enhance the anti-tumor activitv of RSGP.
