Stability of Alkaline in Process of Preparation for Jianghuang Qingzhi Tablets
10.13359/j.cnki.gzxbtcm.2017.02.026
- VernacularTitle:姜黄清脂片制备工艺中碱溶稳定性研究
- Author:
Shanshan HE
;
Haili SUN
;
Min GUO
;
Liping LIAO
;
Xiantao ZHANG
- Keywords:
curcumin;
Jianghuang Qingzhi Tablets/production and preparation;
degradation products;
HPLC-MS;
chromatography,high performance liquid
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(2):260-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the stability of alkaline and degraded products of curcumin by high performance liquid chromatography(HPLC) and HPLC-mass spectrometry (MS) for the optimization of alkaline-dissolved process parameters of the Jianghuang Qingzhi Tablets and for the quality control of the tablets.Methods HPLC was performed for the determination of the alkaline-dissolved stability of curcumin with acetonitrile-acetic acid at volume coefficient of 4％ (48 ∶ 52) as mobile phase,the detection wavelength being 430 nm.The alkalinedegraded products were tested by HPLC-MS assembling with electron spray ionization (ESI) and quadrupole timeof-flight (Q-TOF) in the scan range of 100-2 000 m/z.Results The degradation of curcumin in the alkaline solution was increased with the temperature.When the temperature was below 20 ℃,the degradation slowed down,while when the temperature reached to 80 ℃,curcumin was degraded completely within 2 h.The probable degradation products in the alkaline solution were p-hydroxy benzaldehyde,vanillin,p-coumaric acid,ferulic acid,et al.Conclusion Curcmin compounds are instable in aqueous alkali.To obtain the high-quality of Jianghuang Qingzhi Tablets with high content of curcumin and less degraded products,the alkaline-solution temperature should be controlled below 20 ℃.
