Effect of total saponin of Dioscorea on NALP3 inflammasome signaling pathway with acute gouty in rats
10.3969/j.issn.1001-1978.2017.03.013
- VernacularTitle:萆薢总皂苷对大鼠急性痛风性关节炎NALP3炎性体信号通路的影响
- Author:
Lu WANG
;
Sha NA
;
Guangliang CHEN
- Keywords:
total saponin of Dioscorea;
acute gouty ar-thritis;
MSU;
NALP3;
inflammasome;
inflammatory cytokines
- From:
Chinese Pharmacological Bulletin
2017;33(3):354-360
- CountryChina
- Language:Chinese
-
Abstract:
Aim Toinvestigatetheeffectsoftotalsap-onin of Dioscorea (TSD)on rats with monosodium u-rate crystal-induced acute gouty arthritis (AGA)and mechanisms.Methods Totally72Wistarratswere randomly devided into six groups,Each group was giv-en corresponding drug before,then rat acute gouty ar-thritis model was made by injection of monosodium u-rate in the ankle joint cavity.The gait,articular swell-ing degree and physiological changes of rats were ob-served.The concentration of TNF-α,IL-1β,IL-18 in serum were detected by ELISA.The levels of pro-IL-1β, NALP3, ASC, pro-caspase-1, and cleaved caspase-1 were detected by Western blot.Results AllTSDgroupsandcolchicinesignificantlychangedthe gait of rats and TSD high and middle groups signifi-cantly reduced joint swelling and diminished the patho-logical changes.The levels of TNF-α,IL-1β,IL-18 in serum were significantly decreased,and the levels of pro-IL-1β,NALP3,ASC,pro-caspase-1 and cleaved caspase-1 were apparently reduced in TSD high and middlegroups.Conclusion TSDpossessesanti-goutfunction and the mechanism may be related to sup-pressing the NALP3 inflammasome activation and in-hibiting the cytokine production.
