Effects of gastric bypass surgery on serum total bile acid in obese diabetic patients and rats
10.3969/j.issn.1000-4718.2017.03.013
- VernacularTitle:肥胖2型糖尿病患者与大鼠胃旁路术后血清总胆汁酸水平的变化
- Author:
Weili HE
;
Hening ZHAI
;
Geyang XU
;
Jingge YANG
;
Hua YANG
;
Cunchuan WANG
- Keywords:
Gastric bypass;
Obesity;
Type 2 diabetes mellitus;
Bile acid
- From:
Chinese Journal of Pathophysiology
2017;33(3):462-468
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of gastric bypass surgery on the levels of serum total bile acid in the obese diabetic patients and rats .METHODS: Anthropometric data of obese diabetic patients with gastric bypass surgery from June 2011 to June 2016 were collected in the First Affiliated Hospital of Jinan University .Obese diabetic animal mo-del was established in SD rats by high-fat diet feeding combined with intraperitoneal injection of low-dose streptozocin .Gas-tric bypass surgery or sham operation was performed on the rats with successful modeling .The levels of serum total bile acid were measured by a Hitachi automatic biochemistry analyzer , and the content of hepatic cholesterol 7α-hydroxylase (CYP7A1) was detected by ELISA.The expression of hepatic CYP7A1 and small heterodimer partner (SHP) at mRNA and protein levels was determined by real-time PCR and Western blot, respectively.RESULTS:The serum levels of total bile acid were significantly increased in postoperative obese diabetic patients and rats as compared with control groups .Gas-tric bypass surgery inhibited rat hepatic CYP 7A1 content, mRNA level and protein level , but stimulated hepatic SHP ex-pression.CONCLUSION:Total serum bile acid increases in both patients and rats after gastric bypass surgery by non -typical bile acid synthesis pathway .
