Effects of palmitate-stimulated macrophages on invasion and migration of HepG2 cells
10.3969/j.issn.1000-4718.2017.03.018
- VernacularTitle:棕榈酸刺激的巨噬细胞对HepG2细胞侵袭和迁移的影响
- Author:
Yan WANG
;
Yong YAN
;
Shan ZHONG
;
Xiongzhong RUAN
;
Yaxi CHEN
;
Lei ZHAO
- Keywords:
Palmitate;
HepG2 cells;
Macrophages;
Inflammatory cytokines;
Cell invasion;
Cell migration
- From:
Chinese Journal of Pathophysiology
2017;33(3):495-499
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the impact of palmitate-stimulated macrophages on the invasion and migration of HepG2 cells and to explore the underlying mechanism .METHODS:Human acute monocytic leukemia cell line THP-1 were induced to macrophages by phorbol myristate acetate and were stimulated with palmitate (0.16 mmol/L).The culture supernatants were collected and used to incubate HepG 2 cells.The effect of palmitate on migration of the macrophages was detected by Transwell chamber assay .The mRNA expression of target genes was measured by RT-qPCR.The invasion and migration of the HepG 2 cells were assessed by invasion assay and scratch test .RESULTS:Palmitate promoted the migra-tion of the macrophages and increased the mRNA levels of interleukin -1β( IL-1β) , interleukin-6 ( IL-6 ) , tumor necrosis factor-α(TNF-α) and monocyte chemotactic protein-1 (MCP-1) in the macrophages.The invasion and migration of the HepG2 cells incubated with conditioned media from palmitate-stimulated macrophages were greater than those of the HepG 2 cells incubated with conditioned media from macrophages without palmitate .The media of palmitate-stimulated macrophages up-regulated the mRNA expression of cytokines and N-cadherin, and down-regulated the mRNA expression of E-cadherin in the HepG2 cells.CONCLUSION:Palmitate-stimulated macrophages promote the invasion and migration of HepG 2 cells through paracrine/endcrine loop.
