Mechanism of Shenci capsule combined with cisplatin in reversing DDP resistance of human lung adenocarcinoma via PI3K/AKT/mTOR signa-ling pathway
10.3969/j.issn.1000-4718.2017.03.019
- VernacularTitle:参慈胶囊联合顺铂通过PI3K/AKT/mTOR信号通路逆转人肺腺癌顺铂耐药的机制研究
- Author:
Liqun XU
;
Ronghua ZHANG
;
Ying ZOU
;
Jingjie PAN
;
Xiaodong WU
;
Lijian YU
;
Kun LIANG
- Keywords:
Lung cancer;
Shenci capsule;
DDP resistance;
PI3K/AKT/mTOR signaling pathway;
PTEN;
P-glycoprotein
- From:
Chinese Journal of Pathophysiology
2017;33(3):500-504
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Shenci capsule combined with cisplatin ( DDP) in reversing DDP resistance by PI3K/AKT/mTOR signaling pathway in nude mice bearing A 549/DDP tumor.METHODS:The patient-de-rived lung adenocarcinoma A 549/DDP cell xenograft model was established .The tumor-bearing nude mice were randomly divided into control group , Shenci capsule group , DDP group and Shenci capsule combined with DDP group .The mice in control group was treated with normal saline , while the mice in other groups were treated with different drugs for 21 d.After treatment, the mice were killed and lung cancer tissues were collected .Flow cytometry was used to analyze the cell cycle and apoptosis.FQ-PCR was used to determined the mRNA levels of PTEN , P-glycoprotein, PI3K, AKT and mTOR in A549/DDP lung tumor .RESULTS:Compared with control group , the cell proliferation in all the drug treatment groups was inhibited .Compared with other drug treatment groups , Shenci capsule combined with DDP blocked the cell cycle of A 549/DDP cells at G2/M phase, promoted the apoptosis rate , increased the mRNA expression of PTEN and inhibited the mRNA expression of P-glycoprotein, PI3K, AKT and mTOR.CONCLUSION:Shenci capsule increases the sensitivity of A 549/DDP resistant cells in nude mice to DDP by blocking PI 3K/AKT/mTOR signaling pathway , increasing the expression of PTEN or inhibiting P-glycoprotein-mediated resistance pathway .
