Near full-length genomic analysis of an HIV-1 G subtype strain identified in Guangxi Zhuang Autonomous Region
10.7644/j.issn.1674-9960.2017.02.005
- VernacularTitle:广西1例HIV-1 G亚型毒株全长基因序列的测定和分析
- Author:
Min ZHANG
;
Bingyu LIANG
;
Tianyi LI
;
Ping SHEN
;
Qijian SU
;
Jingyun LI
;
Lin LI
- Keywords:
HIV-1;
G-subtype;
near full-length sequence;
phylogenetic tree analysis
- From:
Military Medical Sciences
2017;41(2):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To sequence and analyze the near full-length genome of an HIV-1 subtype G strain identified in Guangxi,China.Methods The demographic information of an individual infected with HIV-1 subtype G strain was investigated,whose peripherial blood was collected.Viral RNA in plasma was extracted.The near full-length genome of HIV was amplified in two halves using RT-nested-PCR.The PCR products were purified and sequenced.Phylogenetic analysis was made using MEGA6 software.Results A near full-length genome of 8847 bp was obtained.In the neighbor-joining tree,the strain clustered with subtype G references,as supported by the high Bootstrap value (100%).The closest phylogenetic relationship was found between our strain and another subtype G strain (JN106043)previously identified in Guangxi,which was supported by the genetic distance (5%)and high Bootstrap value (100%).Conclusion The strain identified in the study might have originated from subtype G strains in Guangxi,suggesting that subtype G has spread locally in Guangxi.Further surveillance of subtype G epidemic in Guangxi is necessary.The near full-length subtype G strain genome will provide information for the surveillance of HIV in Guangxi.
