Protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd.3 with hypoxic injury
10.3969/j.issn.1000-4718.2017.03.029
- VernacularTitle:活血定眩胶囊含药血清减轻小鼠脑微血管内皮细胞bEnd.3缺氧损伤
- Author:
Tao LIU
;
Min SONG
;
Yanlong GONG
;
Lingtong ZHOU
;
Wantao DONG
;
Jianhong LIU
- Keywords:
Huoxue-Dingxuan capsule;
Mouse brain microvascular endothelial cells;
Hypoxia;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(3):552-556
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd .3 with hypoxic injury .METHODS:The Wistar rats were randomly divided into control group and Huoxue-Dingxuan capsule group .The serum was collected at the 7th day after drug administration . The bEnd.3 cells were divided into normal group , hypoxia serum model group and Huoxue-Dingxuan capsule serum group . After administration of the medicated serum , bEnd.3 cell were treated with hypoxia for 6 h.The cell morphology was ob-served under microscope , the apoptosis rate and cell cycle were detected by flow cytometry , and the activity of superoxide dismutase ( SOD) and the content of malondialdehyde ( MDA) were detected by ELISA .RESULTS:Compared with other groups, Huoxue-Dingxuan capsule-medicated serum significantly resisted the injury caused by hypoxia , obviously improved the morphology of bEnd .3 cells, significantly reduced the number of apoptotic cells induced by hypoxia , and effectively in-hibited the occurrence of hypoxia-induced G1/S phase arrest in the bEnd.3 cells.At the same time, the medicated serum inhibited MDA production , and increased SOD activity .CONCLUSION: Huoxue-Dingxuan capsule attenuates hypoxia-induced bEnd .3 cell damage by enhancing the antioxidant capacity of cells and inhibiting cell apoptosis .
